HegeLab.org

Publications of Tamas Hegedus

Research publications
Book chapters
Conference proceedings
Talks
Posters

Research publications

  1. Hegedus, T., Aleksandrov, A., Mengos, A., Cui, L., Jensen, T.J., Riordan, J.R. Role of individual R domain phosphorylation sites in CFTR regulation by protein kinase A. Biochim Biophys Acta. 2009 Mar 26.
  2. He, L., Aleksandrov, A.A., Serohijos, A.W., Hegedus, T., Aleksandrov, L.A., Cui, L., Dokholyan, N.V., Riordan, J.R. Multiple membrane-cytoplasmic domain contacts in cftr mediate regulation of channel gating. J. Biol. Chem. 2008 Jul 25
  3. Ozvegy-Laczka, C., Laczko, R., Hegedus, C., Litman, T., Varady, G., Goda, K., Hegedus, T., Dokholyan, N.V., Sorrentino, B.P., Varadi, A., Sarkadi, B., Interaction with the 5D3 monoclonal antibody is regulated by intramolecular rearrangements but not by covalent dimer formation of the human ABCG2 multidrug transporter. J. Biol. Chem. 2008 Jul 21;
  4. Hegedus, T., Serohijos, A.W.R., Dokholyan, N.V., He, L., Riordan, J.R., Computational studies reveal phosphorylation-dependent changes in the unstructured R domain of CFTR. J. Mol. Biol. 16, 1052-63
  5. Serohijos, A.W.R., Hegedus, T., Aleksandrov, A., He, L., Cui, L., Dokholyan, N.V., Riordan, J.R., Phenylalanine 508 mediates a cytoplasmic-membrane domain contact in the CFTR 3D structure crucial to assembly and channel function. PNAS, 105, 3256-61
  6. Serohijos, A.W.R., Hegedus, T., Riordan, J.R., Dokholyan, N.V. Diminished self-chaperoning activity of the ΔF508 mutant of CFTR results in protein misfolding. Plos Comp Biol. 4, e1000008; equally contributed
  7. Cui, L., Aleksandrov, L., Chang, X.B., Hou, Y.X., He, L., Hegedus. T., Gentzsch, M., Aleksandrov, A., Balch, W.E., Riordan, J.R. Domain Interdependence in the Biosynthetic Assembly of CFTR. J. Mol. Biol. 2006, Nov 10
  8. Hegedus, T., Aleksandrov, A., Cui, L., Gentzsch, M., Chang X.B., Riordan, J.R. F508del CFTR with two altered RXR motifs escapes from ER quality control but its channel activity is thermally sensitive. BBA Biomembranes, 2006, 1758, 565-72.
  9. Hegedus, T., Riordan, J.R. Search for proteins with similarity to the CFTR R domain using an optimized RDBMS solution mBioSQL. Central European Journal of Biology, 2006, 1, 29-42
  10. Kalmar, L., Hegedus, T., Farkas, H., Nagy, M, Tordai, A. HAEdb: a novel interactive, locus-specific mutation database of the C1 inhibitor gene. Human Mutat., 2004, 25, 1-5
  11. Ozvegy-Laczka, C., Hegedus, T., Varady, G., Ujhelly, O., Schuetz, J.D., Varadi, A., Keri, G., Orfi, L., Nemet, K., Sarkadi, B. High-affinity interaction of tyrosine kinase inhibitors with the ABCG2 multidrug transporter. Mol Pharmacol., 2004, 65,1485-95
  12. Hegedus, T., Sessler, T., Scott, R., Thelin, W., Bakos, E., Varadi, A., Szabo, K., Homolya, L., Milgram, S.L., Sarkadi, B. C-terminal phosphorylation of MRP2 modulates its interaction with PDZ proteins. Biochem Biophys Res Commun., 2003, 302, 454-61
  13. Hegedus, T., Orfi, L., Seprodi, A., Varadi, A., Sarkadi, B., Keri, G. (2002) Interaction of tyrosine kinase inhibitors with the human multidrug transporter proteins, MDR1 and MRP1. Biochimica et Biophysica Acta 1587, 318-325
  14. Szabo, K., Szakacs, G., Hegedus, T., Sarkadi, B. (1999) Nucleotide Occlusion in the Human Cystic Fibrosis Transmembrane Conductance Regulator. J. Biol. Chem. 274, 12209-12212
  15. Hollo, Zs., Homolya, L., Hegedus, T., Muller, M., Szakacs, G., Jakab, K., Antal, F., Sarkadi, B. (1998) Parallel functional and immunological detection of human multidrug resistance proteins, P-glycoprotein and MRP1. Anticancer Research 18(4C):2981-2987
  16. Bakos, E., Hegedus, T., Hollo, Zs., Welker, E., Tusnady, G.E., Varadi, A., Zaman, G., Scheper, R.J., Sarkadi, B. (1996) Membrane Topology and Glycosylation of the Human Multidrug Resistance Associated Protein (MRP). J. Biol. Chem. 271, 12322-12326
  17. Hollo, Zs., Homolya, L., Hegedus, T., Sarkadi, B. (1996) Transport Properties of the Multidrug Resistance Associated Protein (MRP) in Human Tumour Cells. FEBS Letters 383, 99-104

Book chapters

  1. Hegedus, T., Gentzsch, M., Riordan, J.R., CFTR: Understanding the Cause and Influencing the Outcome of Cystic Fibrosis. ABC PROTEINS: From Bacteria to Man, World Scientific Publishing, book chapter - in progress, 2009;
  2. Hegedus, T., Homolya, L., Hollo, Zs., Seprodi, J., Sarkadi, B.: Investigation of human multidrug transporters. IV. Conference of Society of Pro Scientia Gold Medal, 1998, ISBN 963 03 6108 6

Conference proceedings

  1. Serohijos, A.W.R., Hegedus, T., Aleksandrov, A.A., He, L., Cui, L., Dokholyan, N.V., Riordan, J.R., Phenylalanine 508 Forms Intra-domain Contact Crucial To CFTR Folding And Dynamics Biophysical Journal, Volume 96, Issue 3, 382a, 1 February 2009 doi:10.1016/j.bpj.2008.12.2857;
  2. Serohijos, A.W.R., Hegedus, T., Riordan, J.R., Dokholyan, N.V., Molecular origin of increased NBD1-ΔF508 misfolding propensity; 2008, 28th APAMS- Technical Abstracts, p.50;
  3. Remenyi, J., Hegedus, T., Kovacs, P., Csik, G., Sarkadi, B., Hudecz, F., In vitro antitumor effect and localization of daunomycin-polypeptide conjugate in sensitive and resistant cell lines. FEBS J 272: 528- p. (2005);
  4. Hegedus, T., Serohijos, A.W.R., Dokholyan, N.V., He, L., Riordan, J.R., Deciphering CFTR R domain structure and function by computational methods. The 21st Annual North American Cystic Fibrosis Conference, Anaheim, CA, USA, October, 2007; UPDATE REF!
  5. Hudecz, F., Remenyi, J., Hegedus, T., Kovacs, P., Szabo, B., Sarkadi, B., Gaal, D., Peptides: Peptide Revolution: Genomics, Proteomics and Therapeutics. Proceedings of the 18th American Peptide Symposium; Chorev, M.; Sawyer, T. K. Eds.; American Peptide Society, 2004; pp 815;
  6. Hudecz, F., Remenyi, J., Szabo, B., Hegedus, T., Sarkadi, B., Gaal, D. Peptide targeting of daunomycin into sensitive and multidrug resistant tumors. 2003, Biopolymers, 71, 299;
  7. Keri, Gy., Orfi, L., Bokonyi, Gy., Hegedus, T., Hegymegi-Barakonyi, B., Ullrich, A. Sarkadi, B., Development of focused tyrosine kinase inhibitory library and testing against MDR. Peptides 2002. Eds.: E. Benedetti and C. Pedone. Edizioni Ziino, Sorrento, Italy 2002. pp. 530-531;
  8. Remenyi, J., Hegedus, T., Sarkadi, B., Toth, S., Falus, A., Gaal, D., Hudecz, F., Daunomycin-polypetide conjugates: in vitro antitumour effect in sensitive and multidrug resistant cell lines. 2001, Eur J Cancer, 37: 66-;
  9. Remenyi, J., Hegedus, T., Sarkadi, B., Hudecz, F., Effect of a daunomycin-polypeptide conjugate in multidrug resistant tumor cell lines, In: Martinez J, Fehrentz JA (ed.) Peptides 2000: Proceedings of 26th European Peptide Symposium. Paris: Editions EDK, 2001. pp. 941-942. (ISBN:2842540484)
  10. Remenyi, J., Hegedus, T., Sarkadi, B., Hudecz, F., Effect of a daunomycin-polypeptide conjugate in multidrug resistant tumor cell lines. 2002, J Pept Sci 6: 218-;
  11. Homolya, L., Hollo, Zs., Hegedus, T., Sarkadi, B. (1996) Quantitative Characterization of P-glycoprotein Functon (abstract). Cell Biology International 20, 233.

Talks

  1. Hegedus, T., Telbisz, A., Sarkadi, B, Purification of functional ABC transporters for structural studies, Locher-Lab, ETH, Zurich, Switzerland, March, 2011;
  2. Hegedus, T., The role of F508 in the domain folding and in the domain-domain assembly of CFTR, Department of Biophysics, University of Debrecen, Debrecen, Hungary, November, 2010;
  3. Hegedus, T., Structure and dynamics of ABC proteins: interplay of computation and Experiments, Department of Biochemistry, University of Oxford, Oxford, UK, October, 2010;
  4. Gyimesi, G., Ramachandran, S., Kota, P., Dokholyan, N.V., Sarkadi B., Hegedus, T., Computational Study of the Long Scale Dynamics of ABC Proteins. 7th North American ABC meeting, NCI-Frederick, Frederick, MD, USA, September, 2010;
  5. Tordai, H., Ozvegy-Laczka, C., Tusnady, E.G., Aleksandrov, L., Riordan, J.R., Sarkadi, B., Hegedus T., Investigation of membrane topology of ABC proteins using computational and experimental methods, Membrane Transport Conference, Sumeg, Hungary, May of 2010 (oral presentation selected from posters).
  6. Hegedus, T., He, L., Aleksandrov, A., Cui, L., Serohijos, A.W.R., Dokholyan, N.V., Riordan, J.R., CFTR NBD1 folding and domain-domain interactions affected by the ΔF508 mutation. 5th North American ABC meeting, NCI-Frederick, Frederick, MD, USA, September, 2008;
  7. Hegedus, T., He, L., SMFR of CFTR - structure, maturation, function, and regulation. Epithelial Cell Biology Series, UNC, Chapel Hill, September 19, 2008;
  8. Hegedus, T., Aleksandrov, A., Cui, L., Seibert, F., Chang, X.B., Riordan, J.R., Phosphorylation sensitive antibodies as novel tools to study subtle aspects of CFTR R domain function (talk). 3rd North American ABC meeting, NCI-Frederick, Frederick, MD, USA, October, 2006;
  9. Hegedus, T., Aleksandrov, A., Cui, L., Gentzsch, M., Chang, X.B., Riordan, J.R., ΔF508 CFTR with mutations in two arginine-framed tripeptide sequences escapes from ER quality control but remains thermally unstable (talk). 2nd North American ABC meeting, NCI-Frederick, Frederick, MD, USA, September, 2005;
  10. Hegedus, T.: Resistance to cancer chemotherapy: drug and PDZ protein interactions of ABC transporters. Mayo Clinic, Scottsdale, Arizona, October 8, 2002;
  11. Hegedus, T., Sessler, T., Scott, R., William, T., Bakos, E., Varadi, A., Homolya, L., Milgram, S.E., Sarkadi, B.: Interaction of PDZ proteins with the human MRP2 protein - possible modulation by phosphorylation. Forefronts in Nephrology: ABC Proteins in Epitherlial Physiology, Ascona, Switzerland, April 20-25, 2002;
  12. Hegedus, T., Sessler, T., Milgram, S.E., Sarkadi, B.: Investigation of interactions among ABC transporters and PDZ proteins. 10th Cell biological and developmental meeting, Debrecen, Hungary, January 21-24, 2001.;
  13. Hegedus, T.: Investigation of interactions among ABC transporters and PDZ proteins. Dept. of Moelcular Physiology, UNC, Chapel Hill, January 15, 2001;
  14. Hegedus, T., Sessler, T., Milgram, S.E., Sarkadi, B.: Investigation of interactions among ABC transporters and PDZ proteins. V. Conference of Society of Pro Scientia Gold Medal, Sopron, Hungary, Novermber 4-7, 2000;
  15. Hegedus, T., Homolya, L., Hollo, Zs., Seprodi, J., Sarkadi, B.: Studying of function of human MDR proteins. IV. Conference of Society of Pro Scientia Gold Medal, Szeged, Hungary, November 6-7, 1998;
  16. Hegedus, T., Muller, M., Hollo Zs., Sarkadi, B.: Separation of P-glycoprotein and MRP Expressing Tumor Cells by Single Cell Fluorescence Imaging. Conference of Hungarian Society of Biophysics, Pecs, Hungary, July 6-8, 1997
  17. Hegedus, T., Homolya, L., Muller, M., Hollo, Zs., Sarkadi, B.: Studying of function of MDR proteins. National Scientific Conference for Students, Nyiregyhaza, Hungary, March 24-26, 1997;
  18. Hegedus, T., Homolya, L., Hollo, Zs., Sarkadi, B.: Quantitative Characterization of P-glycoprotein Functon. Scientific Conference for Students, Budapest, Hungary, March 28-29, 1996.
Posters
  1. Tordai, H., Ozvegy-Laczka, C., Tusnady, E.G., Aleksandrov, L., Riordan, J.R., Sarkadi, B, Hegedus T., Investigation of membrane topology of ABC proteins using computational and experimental methods, Membrane Transport Conference, Sumeg, Hungary, May of 2010;
  2. Gyimesi, G., Ramachandran, S., Kota, P., Dokholyan, N.V., Sarkadi, B., Hegedus, T., Characterizing the dynamics of different conformers of ABC transporters. Membrane Transport Conference, Sumeg, Hungary, May of 2010;
  3. Gyimesi, G., Ramachandran, S., Kota, P., Dokholyan, N.V., Sarkadi, B., Hegedus, T., Characterizing the dynamics of different conformers of ABC transporters. ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Disease, Innsbruck, Austria, 2010 (in the 15 best posters out of ~200).
  4. Hegedus, T., Serohijos, A.W.R., Dokholyan, N.V., He, L., Riordan, J.R., Structural characterization of the disordered R domain suggests a mechanism of CFTR regulation. 6th FEBS Advanced Lecture Course "ATP-binding Cassette Transporters: From Multidrug Resistance to Genetic Disease", Innsbruck, Austria, March, 2008;
  5. Hegedus, T., Aleksandrov, A., Cui, L., Seibert, F., Chang, X.B., Riordan, J.R., New tools and a novel model based on disorder predictors to study and understand CFTR R domain function. 4th FEBS Advanced Lecture Course "ATP-binding Cassette Transporters: From Multidrug Resistance to Genetic Disease", Innsbruck, Austria, March, 2006;
  6. Hegedus, T., Aleksandrov, A., Cui, L., Chang, X.B., Riordan, J.R., ΔF508 CFTR remains thermally unstable after escape from ER quality control due to mutation of two arginine-framed tripeptide sequences. 30th FEBS Congress and 9th IUBMB Conference, Budapest, Hungary, July, 2005;
  7. Hegedus, T., Sessler, T., Homolya, L., Milgram, S.E., Sarkadi, B.: Phosphorylation of MRP2 C-terminus modulates its interaction with PDZ proteins. 4th FEBS Advanced Lecture Course "ATP-binding Cassette Transporters: From Multidrug Resistance to Genetic Disease", Gosau, Austria, March 1-8, 2003;
  8. Hegedus, T., Sessler, T., Scott, R., William, T., Bakos, E., Varadi, A., Homolya, L., Milgram, S.E., Sarkadi, B.: Investigation of PDZ interactions of CFTR, MRP2, and ABCA1 proteins. 7th Conference of the Molecular Biology Section of Hungarian Biochemical Society,Keszthely, Hungary, May 14-17, 2002;
  9. Hegedus, T., Scott, R., Sessler, T., Szabo, K., Bakos, E., Milgram, S.E., Sarkadi, B.: Interactions between ABC transporters and PDZ proteins. 3rd FEBS Advanced Lecture Course "ATP-binding Cassette Transporters: From Multidrug Resistance to Genetic Disease", Gosau, Austria, March 3-10, 2001;
  10. Hegedus, T., R., Sessler, T., Szabo, K., Bakos, E., Milgram, S.E., Sarkadi, B.: Interaction of CFTR and MRP2 with PDZ proteins. 6th Conference of the Molecular Biology Section of Hungarian Biochemical Society, May 14-17, 2001;
  11. Hegedus, T., Keri, Gy., Orffy, L., Sarkadi, B.: Effect of tyrosin kinase inhibitors on human multidrug resistance proteins. 2nd FEBS Advanced Lecture Course "ATP-binding Cassette Transporters: From Multidrug Resistance to Genetic Disease", Gosau, Austria, February 20-27, 1999;
  12. Hegedus, T., Muller, M., Hollo, Zs., Sarkadi, B.: Parallel functional and immunological detection of human multidrug resistance proteins, P-glycoprotein and MRP1. Conference on Membrane Transport, Sumeg, Hungary, May 20-23, 1997;
  13. Hegedus, T., Muller, M., Hollo, Zs., Sarkadi, B.: Separation of P-glycoprotein and MRP expressing tumor cells by single cell fluorescence imaging. FEBS advanced Lecture Course "ATP Binding Cassette (ABC) Transporters: From Multidrug Resistance to Genetic Disease", Gosau, Austria, March 1- February 22, 1997;
  14. Hegedus, T., Homolya, L., Hollo, Zs., Sarkadi, B.: Kinetic Study of Pgp-mediated Drug-Transport. 24th FEBS Meeting, Barcelona, Spain, 7-12 July 1996.