Publications of Tamas Hegedus
Research publications
Book chapters
Conference proceedings
Talks
Posters
Research publications
- Hegedus, T., Aleksandrov, A., Mengos, A., Cui, L., Jensen, T.J., Riordan, J.R. Role of individual R domain phosphorylation sites in CFTR regulation by protein kinase A. Biochim Biophys Acta. 2009 Mar 26.
- He, L., Aleksandrov, A.A., Serohijos, A.W., Hegedus, T., Aleksandrov, L.A., Cui, L., Dokholyan, N.V., Riordan, J.R. Multiple membrane-cytoplasmic domain contacts in cftr mediate regulation of channel gating. J. Biol. Chem. 2008 Jul 25
- Ozvegy-Laczka, C., Laczko, R., Hegedus, C., Litman, T., Varady, G., Goda, K., Hegedus, T., Dokholyan, N.V., Sorrentino, B.P., Varadi, A., Sarkadi, B., Interaction with the 5D3 monoclonal antibody is regulated by intramolecular rearrangements but not by covalent dimer formation of the human ABCG2 multidrug transporter. J. Biol. Chem. 2008 Jul 21;
- Hegedus, T., Serohijos, A.W.R., Dokholyan, N.V., He, L., Riordan, J.R., Computational studies reveal phosphorylation-dependent changes in the unstructured R domain of CFTR. J. Mol. Biol. 16, 1052-63
- Serohijos, A.W.R., Hegedus, T., Aleksandrov, A., He, L., Cui, L., Dokholyan, N.V., Riordan, J.R., Phenylalanine 508 mediates a cytoplasmic-membrane domain contact in the CFTR 3D structure crucial to assembly and channel function. PNAS, 105, 3256-61
- Serohijos, A.W.R., Hegedus, T., Riordan, J.R., Dokholyan, N.V. Diminished self-chaperoning activity of the ΔF508 mutant of CFTR results in protein misfolding. Plos Comp Biol. 4, e1000008; equally contributed
- Cui, L., Aleksandrov, L., Chang, X.B., Hou, Y.X., He, L., Hegedus. T., Gentzsch, M., Aleksandrov, A., Balch, W.E., Riordan, J.R. Domain Interdependence in the Biosynthetic Assembly of CFTR. J. Mol. Biol. 2006, Nov 10
- Hegedus, T., Aleksandrov, A., Cui, L., Gentzsch, M., Chang X.B., Riordan, J.R. F508del CFTR with two altered RXR motifs escapes from ER quality control but its channel activity is thermally sensitive. BBA Biomembranes, 2006, 1758, 565-72.
- Hegedus, T., Riordan, J.R. Search for proteins with similarity to the CFTR R domain using an optimized RDBMS solution mBioSQL. Central European Journal of Biology, 2006, 1, 29-42
- Kalmar, L., Hegedus, T., Farkas, H., Nagy, M, Tordai, A. HAEdb: a novel interactive, locus-specific mutation database of the C1 inhibitor gene. Human Mutat., 2004, 25, 1-5
- Ozvegy-Laczka, C., Hegedus, T., Varady, G., Ujhelly, O., Schuetz, J.D., Varadi, A., Keri, G., Orfi, L., Nemet, K., Sarkadi, B. High-affinity interaction of tyrosine kinase inhibitors with the ABCG2 multidrug transporter. Mol Pharmacol., 2004, 65,1485-95
- Hegedus, T., Sessler, T., Scott, R., Thelin, W., Bakos, E., Varadi, A., Szabo, K., Homolya, L., Milgram, S.L., Sarkadi, B. C-terminal phosphorylation of MRP2 modulates its interaction with PDZ proteins. Biochem Biophys Res Commun., 2003, 302, 454-61
- Hegedus, T., Orfi, L., Seprodi, A., Varadi, A., Sarkadi, B., Keri, G. (2002) Interaction of tyrosine kinase inhibitors with the human multidrug transporter proteins, MDR1 and MRP1. Biochimica et Biophysica Acta 1587, 318-325
- Szabo, K., Szakacs, G., Hegedus, T., Sarkadi, B. (1999) Nucleotide Occlusion in the Human Cystic Fibrosis Transmembrane Conductance Regulator. J. Biol. Chem. 274, 12209-12212
- Hollo, Zs., Homolya, L., Hegedus, T., Muller, M., Szakacs, G., Jakab, K., Antal, F., Sarkadi, B. (1998) Parallel functional and immunological detection of human multidrug resistance proteins, P-glycoprotein and MRP1. Anticancer Research 18(4C):2981-2987
- Bakos, E., Hegedus, T., Hollo, Zs., Welker, E., Tusnady, G.E., Varadi, A., Zaman, G., Scheper, R.J., Sarkadi, B. (1996) Membrane Topology and Glycosylation of the Human Multidrug Resistance Associated Protein (MRP). J. Biol. Chem. 271, 12322-12326
- Hollo, Zs., Homolya, L., Hegedus, T., Sarkadi, B. (1996) Transport Properties of the Multidrug Resistance Associated Protein (MRP) in Human Tumour Cells. FEBS Letters 383, 99-104
Book chapters
- Hegedus, T., Gentzsch, M., Riordan, J.R., CFTR: Understanding the Cause and Influencing the Outcome of Cystic Fibrosis. ABC PROTEINS: From Bacteria to Man, World Scientific Publishing, book chapter - in progress, 2009;
- Hegedus, T., Homolya, L., Hollo, Zs., Seprodi, J., Sarkadi, B.: Investigation of human multidrug transporters. IV. Conference of Society of Pro Scientia Gold Medal, 1998, ISBN 963 03 6108 6
Conference proceedings
- Serohijos, A.W.R., Hegedus, T., Aleksandrov, A.A., He, L., Cui, L., Dokholyan, N.V., Riordan, J.R., Phenylalanine 508 Forms Intra-domain Contact Crucial To CFTR Folding And Dynamics Biophysical Journal, Volume 96, Issue 3, 382a, 1 February 2009 doi:10.1016/j.bpj.2008.12.2857;
- Serohijos, A.W.R., Hegedus, T., Riordan, J.R., Dokholyan, N.V., Molecular origin of increased NBD1-ΔF508 misfolding propensity; 2008, 28th APAMS- Technical Abstracts, p.50;
- Remenyi, J., Hegedus, T., Kovacs, P., Csik, G., Sarkadi, B., Hudecz, F., In vitro antitumor effect and localization of daunomycin-polypeptide conjugate in sensitive and resistant cell lines. FEBS J 272: 528- p. (2005);
- Hegedus, T., Serohijos, A.W.R., Dokholyan, N.V., He, L., Riordan, J.R., Deciphering CFTR R domain structure and function by computational methods. The 21st Annual North American Cystic Fibrosis Conference, Anaheim, CA, USA, October, 2007; UPDATE REF!
- Hudecz, F., Remenyi, J., Hegedus, T., Kovacs, P., Szabo, B., Sarkadi, B., Gaal, D., Peptides: Peptide Revolution: Genomics, Proteomics and Therapeutics. Proceedings of the 18th American Peptide Symposium; Chorev, M.; Sawyer, T. K. Eds.; American Peptide Society, 2004; pp 815;
- Hudecz, F., Remenyi, J., Szabo, B., Hegedus, T., Sarkadi, B., Gaal, D. Peptide targeting of daunomycin into sensitive and multidrug resistant tumors. 2003, Biopolymers, 71, 299;
- Keri, Gy., Orfi, L., Bokonyi, Gy., Hegedus, T., Hegymegi-Barakonyi, B., Ullrich, A. Sarkadi, B., Development of focused tyrosine kinase inhibitory library and testing against MDR. Peptides 2002. Eds.: E. Benedetti and C. Pedone. Edizioni Ziino, Sorrento, Italy 2002. pp. 530-531;
- Remenyi, J., Hegedus, T., Sarkadi, B., Toth, S., Falus, A., Gaal, D., Hudecz, F., Daunomycin-polypetide conjugates: in vitro antitumour effect in sensitive and multidrug resistant cell lines. 2001, Eur J Cancer, 37: 66-;
- Remenyi, J., Hegedus, T., Sarkadi, B., Hudecz, F., Effect of a daunomycin-polypeptide conjugate in multidrug resistant tumor cell lines, In: Martinez J, Fehrentz JA (ed.) Peptides 2000: Proceedings of 26th European Peptide Symposium. Paris: Editions EDK, 2001. pp. 941-942. (ISBN:2842540484)
- Remenyi, J., Hegedus, T., Sarkadi, B., Hudecz, F., Effect of a daunomycin-polypeptide conjugate in multidrug resistant tumor cell lines. 2002, J Pept Sci 6: 218-;
- Homolya, L., Hollo, Zs., Hegedus, T., Sarkadi, B. (1996) Quantitative Characterization of P-glycoprotein Functon (abstract). Cell Biology International 20, 233.
Talks
- Hegedus, T., Telbisz, A., Sarkadi, B, Purification of functional ABC transporters for structural studies, Locher-Lab, ETH, Zurich, Switzerland, March, 2011;
- Hegedus, T., The role of F508 in the domain folding and in the domain-domain assembly of CFTR, Department of Biophysics, University of Debrecen, Debrecen, Hungary, November, 2010;
- Hegedus, T., Structure and dynamics of ABC proteins: interplay of computation and Experiments, Department of Biochemistry, University of Oxford, Oxford, UK, October, 2010;
- Gyimesi, G., Ramachandran, S., Kota, P., Dokholyan, N.V., Sarkadi B., Hegedus, T., Computational Study of the Long Scale Dynamics of ABC Proteins. 7th North American ABC meeting, NCI-Frederick, Frederick, MD, USA, September, 2010;
- Tordai, H., Ozvegy-Laczka, C., Tusnady, E.G., Aleksandrov, L., Riordan, J.R., Sarkadi, B., Hegedus T., Investigation of membrane topology of ABC proteins using computational and experimental methods, Membrane Transport Conference, Sumeg, Hungary, May of 2010 (oral presentation selected from posters).
- Hegedus, T., He, L., Aleksandrov, A., Cui, L., Serohijos, A.W.R., Dokholyan, N.V., Riordan, J.R., CFTR NBD1 folding and domain-domain interactions affected by the ΔF508 mutation. 5th North American ABC meeting, NCI-Frederick, Frederick, MD, USA, September, 2008;
- Hegedus, T., He, L., SMFR of CFTR - structure, maturation, function, and regulation. Epithelial Cell Biology Series, UNC, Chapel Hill, September 19, 2008;
- Hegedus, T., Aleksandrov, A., Cui, L., Seibert, F., Chang, X.B., Riordan, J.R., Phosphorylation sensitive antibodies as novel tools to study subtle aspects of CFTR R domain function (talk). 3rd North American ABC meeting, NCI-Frederick, Frederick, MD, USA, October, 2006;
- Hegedus, T., Aleksandrov, A., Cui, L., Gentzsch, M., Chang, X.B., Riordan, J.R., ΔF508 CFTR with mutations in two arginine-framed tripeptide sequences escapes from ER quality control but remains thermally unstable (talk). 2nd North American ABC meeting, NCI-Frederick, Frederick, MD, USA, September, 2005;
- Hegedus, T.: Resistance to cancer chemotherapy: drug and PDZ protein interactions of ABC transporters. Mayo Clinic, Scottsdale, Arizona, October 8, 2002;
- Hegedus, T., Sessler, T., Scott, R., William, T., Bakos, E., Varadi, A., Homolya, L., Milgram, S.E., Sarkadi, B.: Interaction of PDZ proteins with the human MRP2 protein - possible modulation by phosphorylation. Forefronts in Nephrology: ABC Proteins in Epitherlial Physiology, Ascona, Switzerland, April 20-25, 2002;
- Hegedus, T., Sessler, T., Milgram, S.E., Sarkadi, B.: Investigation of interactions among ABC transporters and PDZ proteins. 10th Cell biological and developmental meeting, Debrecen, Hungary, January 21-24, 2001.;
- Hegedus, T.: Investigation of interactions among ABC transporters and PDZ proteins. Dept. of Moelcular Physiology, UNC, Chapel Hill, January 15, 2001;
- Hegedus, T., Sessler, T., Milgram, S.E., Sarkadi, B.: Investigation of interactions among ABC transporters and PDZ proteins. V. Conference of Society of Pro Scientia Gold Medal, Sopron, Hungary, Novermber 4-7, 2000;
- Hegedus, T., Homolya, L., Hollo, Zs., Seprodi, J., Sarkadi, B.: Studying of function of human MDR proteins. IV. Conference of Society of Pro Scientia Gold Medal, Szeged, Hungary, November 6-7, 1998;
- Hegedus, T., Muller, M., Hollo Zs., Sarkadi, B.: Separation of P-glycoprotein and MRP Expressing Tumor Cells by Single Cell Fluorescence Imaging. Conference of Hungarian Society of Biophysics, Pecs, Hungary, July 6-8, 1997
- Hegedus, T., Homolya, L., Muller, M., Hollo, Zs., Sarkadi, B.: Studying of function of MDR proteins. National Scientific Conference for Students, Nyiregyhaza, Hungary, March 24-26, 1997;
- Hegedus, T., Homolya, L., Hollo, Zs., Sarkadi, B.: Quantitative Characterization of P-glycoprotein Functon. Scientific Conference for Students, Budapest, Hungary, March 28-29, 1996.
- Tordai, H., Ozvegy-Laczka, C., Tusnady, E.G., Aleksandrov, L., Riordan, J.R., Sarkadi, B, Hegedus T., Investigation of membrane topology of ABC proteins using computational and experimental methods, Membrane Transport Conference, Sumeg, Hungary, May of 2010;
- Gyimesi, G., Ramachandran, S., Kota, P., Dokholyan, N.V., Sarkadi, B., Hegedus, T., Characterizing the dynamics of different conformers of ABC transporters. Membrane Transport Conference, Sumeg, Hungary, May of 2010;
- Gyimesi, G., Ramachandran, S., Kota, P., Dokholyan, N.V., Sarkadi, B., Hegedus, T., Characterizing the dynamics of different conformers of ABC transporters. ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Disease, Innsbruck, Austria, 2010 (in the 15 best posters out of ~200).
- Hegedus, T., Serohijos, A.W.R., Dokholyan, N.V., He, L., Riordan, J.R., Structural characterization of the disordered R domain suggests a mechanism of CFTR regulation. 6th FEBS Advanced Lecture Course "ATP-binding Cassette Transporters: From Multidrug Resistance to Genetic Disease", Innsbruck, Austria, March, 2008;
- Hegedus, T., Aleksandrov, A., Cui, L., Seibert, F., Chang, X.B., Riordan, J.R., New tools and a novel model based on disorder predictors to study and understand CFTR R domain function. 4th FEBS Advanced Lecture Course "ATP-binding Cassette Transporters: From Multidrug Resistance to Genetic Disease", Innsbruck, Austria, March, 2006;
- Hegedus, T., Aleksandrov, A., Cui, L., Chang, X.B., Riordan, J.R., ΔF508 CFTR remains thermally unstable after escape from ER quality control due to mutation of two arginine-framed tripeptide sequences. 30th FEBS Congress and 9th IUBMB Conference, Budapest, Hungary, July, 2005;
- Hegedus, T., Sessler, T., Homolya, L., Milgram, S.E., Sarkadi, B.: Phosphorylation of MRP2 C-terminus modulates its interaction with PDZ proteins. 4th FEBS Advanced Lecture Course "ATP-binding Cassette Transporters: From Multidrug Resistance to Genetic Disease", Gosau, Austria, March 1-8, 2003;
- Hegedus, T., Sessler, T., Scott, R., William, T., Bakos, E., Varadi, A., Homolya, L., Milgram, S.E., Sarkadi, B.: Investigation of PDZ interactions of CFTR, MRP2, and ABCA1 proteins. 7th Conference of the Molecular Biology Section of Hungarian Biochemical Society,Keszthely, Hungary, May 14-17, 2002;
- Hegedus, T., Scott, R., Sessler, T., Szabo, K., Bakos, E., Milgram, S.E., Sarkadi, B.: Interactions between ABC transporters and PDZ proteins. 3rd FEBS Advanced Lecture Course "ATP-binding Cassette Transporters: From Multidrug Resistance to Genetic Disease", Gosau, Austria, March 3-10, 2001;
- Hegedus, T., R., Sessler, T., Szabo, K., Bakos, E., Milgram, S.E., Sarkadi, B.: Interaction of CFTR and MRP2 with PDZ proteins. 6th Conference of the Molecular Biology Section of Hungarian Biochemical Society, May 14-17, 2001;
- Hegedus, T., Keri, Gy., Orffy, L., Sarkadi, B.: Effect of tyrosin kinase inhibitors on human multidrug resistance proteins. 2nd FEBS Advanced Lecture Course "ATP-binding Cassette Transporters: From Multidrug Resistance to Genetic Disease", Gosau, Austria, February 20-27, 1999;
- Hegedus, T., Muller, M., Hollo, Zs., Sarkadi, B.: Parallel functional and immunological detection of human multidrug resistance proteins, P-glycoprotein and MRP1. Conference on Membrane Transport, Sumeg, Hungary, May 20-23, 1997;
- Hegedus, T., Muller, M., Hollo, Zs., Sarkadi, B.: Separation of P-glycoprotein and MRP expressing tumor cells by single cell fluorescence imaging. FEBS advanced Lecture Course "ATP Binding Cassette (ABC) Transporters: From Multidrug Resistance to Genetic Disease", Gosau, Austria, March 1- February 22, 1997;
- Hegedus, T., Homolya, L., Hollo, Zs., Sarkadi, B.: Kinetic Study of Pgp-mediated Drug-Transport. 24th FEBS Meeting, Barcelona, Spain, 7-12 July 1996.